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Evaluation of the ASCL1 knockdown and its effects on CRC factors by <t>Sh6</t> and <t>Sh8</t> in Kelly Sh6 and Sh8 induced knockdown of ASCL1 in the presence of doxycycline (Dox) at day 4, as assessed by (A and B) RT-PCR and (C and D) western blot analysis. (E and F) Gene expression of ASCL1, SOX10, and GATA3 in Sh6- and Sh8-inducible Kelly cells by RT-PCR analysis. (G and H) Protein expression of CRC transcription factors in Sh6 and Sh8 cells. (All statistical data presented as mean ± SEM with n = 3, ∗∗p < 0.01 or ∗p < 0.05.)
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Evaluation of the ASCL1 knockdown and its effects on CRC factors by <t>Sh6</t> and <t>Sh8</t> in Kelly Sh6 and Sh8 induced knockdown of ASCL1 in the presence of doxycycline (Dox) at day 4, as assessed by (A and B) RT-PCR and (C and D) western blot analysis. (E and F) Gene expression of ASCL1, SOX10, and GATA3 in Sh6- and Sh8-inducible Kelly cells by RT-PCR analysis. (G and H) Protein expression of CRC transcription factors in Sh6 and Sh8 cells. (All statistical data presented as mean ± SEM with n = 3, ∗∗p < 0.01 or ∗p < 0.05.)
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Evaluation of the ASCL1 knockdown and its effects on CRC factors by <t>Sh6</t> and <t>Sh8</t> in Kelly Sh6 and Sh8 induced knockdown of ASCL1 in the presence of doxycycline (Dox) at day 4, as assessed by (A and B) RT-PCR and (C and D) western blot analysis. (E and F) Gene expression of ASCL1, SOX10, and GATA3 in Sh6- and Sh8-inducible Kelly cells by RT-PCR analysis. (G and H) Protein expression of CRC transcription factors in Sh6 and Sh8 cells. (All statistical data presented as mean ± SEM with n = 3, ∗∗p < 0.01 or ∗p < 0.05.)
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Evaluation of the ASCL1 knockdown and its effects on CRC factors by <t>Sh6</t> and <t>Sh8</t> in Kelly Sh6 and Sh8 induced knockdown of ASCL1 in the presence of doxycycline (Dox) at day 4, as assessed by (A and B) RT-PCR and (C and D) western blot analysis. (E and F) Gene expression of ASCL1, SOX10, and GATA3 in Sh6- and Sh8-inducible Kelly cells by RT-PCR analysis. (G and H) Protein expression of CRC transcription factors in Sh6 and Sh8 cells. (All statistical data presented as mean ± SEM with n = 3, ∗∗p < 0.01 or ∗p < 0.05.)
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Evaluation of the ASCL1 knockdown and its effects on CRC factors by Sh6 and Sh8 in Kelly Sh6 and Sh8 induced knockdown of ASCL1 in the presence of doxycycline (Dox) at day 4, as assessed by (A and B) RT-PCR and (C and D) western blot analysis. (E and F) Gene expression of ASCL1, SOX10, and GATA3 in Sh6- and Sh8-inducible Kelly cells by RT-PCR analysis. (G and H) Protein expression of CRC transcription factors in Sh6 and Sh8 cells. (All statistical data presented as mean ± SEM with n = 3, ∗∗p < 0.01 or ∗p < 0.05.)

Journal: Molecular Therapy Oncolytics

Article Title: Therapeutically targeting oncogenic CRCs facilitates induced differentiation of NB by RA and the BET bromodomain inhibitor

doi: 10.1016/j.omto.2021.09.004

Figure Lengend Snippet: Evaluation of the ASCL1 knockdown and its effects on CRC factors by Sh6 and Sh8 in Kelly Sh6 and Sh8 induced knockdown of ASCL1 in the presence of doxycycline (Dox) at day 4, as assessed by (A and B) RT-PCR and (C and D) western blot analysis. (E and F) Gene expression of ASCL1, SOX10, and GATA3 in Sh6- and Sh8-inducible Kelly cells by RT-PCR analysis. (G and H) Protein expression of CRC transcription factors in Sh6 and Sh8 cells. (All statistical data presented as mean ± SEM with n = 3, ∗∗p < 0.01 or ∗p < 0.05.)

Article Snippet: Two different mission shRNAs from the TRC1 library (Sigma-Aldrich; TRCN0000013550 and TRCN0000235656, referred to in the manuscript as sh6 and sh8, respectively) targeting ASCL1 and one non-targeting shRNA control (NTC) were used to generate Kelly NBCLs with ASCL1 knockdown.

Techniques: Reverse Transcription Polymerase Chain Reaction, Western Blot, Expressing

Effect of ASCL1 knockdown and treatment of ATRA on proliferation and differentiation (A and B) The gene expression of ASCL1 and TH in day-3 treatments of Dox versus Dox + ATRA in Sh6- and Sh8-inducible cells by RT-PCR assay. (C) Protein expression of ASCL1 and TH as assessed by western blots. (D) Proliferation percentage in Sh6- and Sh8-inducible cells at day-4 treatment of Dox and Dox + ATRA combination as measured by CyQuant (n = 3, ∗∗p < 0.01 or ∗p < 0.05).

Journal: Molecular Therapy Oncolytics

Article Title: Therapeutically targeting oncogenic CRCs facilitates induced differentiation of NB by RA and the BET bromodomain inhibitor

doi: 10.1016/j.omto.2021.09.004

Figure Lengend Snippet: Effect of ASCL1 knockdown and treatment of ATRA on proliferation and differentiation (A and B) The gene expression of ASCL1 and TH in day-3 treatments of Dox versus Dox + ATRA in Sh6- and Sh8-inducible cells by RT-PCR assay. (C) Protein expression of ASCL1 and TH as assessed by western blots. (D) Proliferation percentage in Sh6- and Sh8-inducible cells at day-4 treatment of Dox and Dox + ATRA combination as measured by CyQuant (n = 3, ∗∗p < 0.01 or ∗p < 0.05).

Article Snippet: Two different mission shRNAs from the TRC1 library (Sigma-Aldrich; TRCN0000013550 and TRCN0000235656, referred to in the manuscript as sh6 and sh8, respectively) targeting ASCL1 and one non-targeting shRNA control (NTC) were used to generate Kelly NBCLs with ASCL1 knockdown.

Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Western Blot, CyQUANT Assay